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Tissue Culture Lab

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Carnivorous Plants

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SUNDEW

Drosera communis

VENUS FLY TRAP

Dionaea muscipula 'B52'

BUTTERWORT
Pinguicula moranensis

RAINBOW PLANT
Byblis guehoi

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PITCHER PLANT 
Nepenthes Hybrid (Thailand)

Laboratory Materials

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Erlenmeyer Flask

Glassware

Specifications:

- Pyrex Brand

- Clear ConicalGlass

- 500 ml Capacity

- Borosilicate Glass

- Autoclavable

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Top Diameter: 4 cm/ 1.57 inches

Bottom Diameter: 7 cm/ 2.76 inches

Height: 18.5 cm/ 7.28 inches

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Uses: For plant tissue culture, microbiological, and any laboratory-related analysis and experimentation.

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Graduated Cylinder

Glassware

Specifications:

- Bomex Brand

- Clear Glass

- 100 ml Capacity

- Accurate volume measurement up to +/- 1 cm

- Approximately 25 cm tall and 3 cm diameter

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Uses: For measuring the specific volume of liquid chemicals for plant tissue culture and any laboratory-related experiments. 

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Petri Dish

Glassware

Specifications:

- Unbranded

- Clear Quartz Glass; Thick

- Borosilicate

- Autoclavable

- 90 mm wide

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Uses: For inoculation and subculturing of specimen for plant tissue culture. For microbiological analysis of bacterium and fungus as well as for cell culture (plants and animals).

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Alcohol Lamp

Glassware

Specifications:

- Unbranded

- High-quality glass lamp with cover.

- 50 ml Capacity

- Minimal Space (for safety purposes).

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Uses: For sterilizing/sanitizing plant tissue culture and microbiological apparatus such as forceps, scalpels, wire inoculating loop, etc. 

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Long Forceps

Tissue Culture Utensils

Specifications:

- Pointed/Elbow Type Forceps

- Stainless Steel

- 28 cm Long 

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Uses: For plant tissue culture inoculation and subculturing, aquarium landscape and repotting of small plants.

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Short Forceps

Tissue Culture Utensils

Specifications 

- Angled Clamped Forceps

- Stainless Steel

- 23 cm Long

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Uses: A medical/surgical instrument that is also used for plant tissue culture inoculation and subculturing, and repotting of small plants. 

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Scalpel Holder

Tissue Culture Utensils

Specifications:

- Scalpel Handle No. 4 

- Compatibility Blades: #20, #21, #22, #23

- Stainless Steel

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Uses: For plant tissue culture inoculation and can also be used for plant and animal dissection. 

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Scalpel Blade

Tissue Culture Utensils

Specifications: 

- Sterile Blade #22

- Compatible Scalpel Holder #4

- 10 pcs per pack 

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Uses: For plant tissue culture inoculation and can also be used for plant and animal dissection.

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Alpha Naphthalene Acetic Acid/ANAA

Plant Growth Regulator/Hormone

Specifications: 

- Auxin 98% Purity

- White Fine Powder

- Highly Concentrated Rooting Hormone

- 3 g per pack (equivalent to 3,000 ppm)

- Soluble in ethyl alcohol, potassium hydroxide, and sodium hydroxide 1N (1 Normal).

- For rooting and seed germination in vitro.

- Store in a cool dry place.

- Do not place under direct sunlight.

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Indole-3 Butyric Acid/IBA 99%

Plant Growth Regulator/Hormone

Specifications:

- Auxin 99% Purity

- Off-White Fine Powder

- Highly Concentrated Rooting Hormone

- 3 g per pack (equivalent to 3,000 ppm)

- Soluble in ethyl alcohol, potassium hydroxide, and sodium hydroxide 1N (1 Normal)

- For rooting and seed germination in vitro.

- Store in a cool dry place.

- Do not direct from sunlight.

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6-Benzylaminopurine/6-BAP

Plant Growth Regulator/Hormone

Specifications: 

- Cytokinin 98% Purity

- White Fine Powder

- Highly Concentrated Shooting Hormone

- Soluble in ethyl alcohol, potassium hydroxide, and sodium hydroxide 1N (1 Normal).

- For cloning/multiple plant inducer in vitro.

- Store in a cool dry place.

- Do not place under direct sunlight.

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Thidiazuron/TDZ

Plant Growth Regulator/Hormone

Specifications:

- Cytokinin 95%

- Yellow Fine Powder

- Highly Concentrated Shooting Hormone

- Soluble in ethyl alcohol, potassium hydroxide, and sodium hydroxide 1N (1 Normal)

- For cloning, multiple plant inducer, plant cell division in vitro

- Store in a cool dry place.

- Keep away from direct sunlight.

- Keep away from children.

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95% Ethyl Alcohol

Chemicals

Specifications:
- 1 litre
- Transparent Liquid
- Sanitizing Agent
- Solvent for plant growth hormones
- Can also be diluted to make 70% alcohol.
- Used as a flaming substance in tissue culture (blue flame).
- Store in a cool dry place.
- Keep away from direct sunlight.
- Keep away from children.

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Potassium Hydroxide/KOH

Chemicals

Specifications:

- Technical Grade

- pH Up Solution 

- White or slightly yellow flakes, odorless.

- Available in 120 ml diluted pH adjuster and 60 g flakes good for 1 L (equivalent to 1 Normal solution).

- Corrosive. If your skin comes into contact with KOH, place a few drops of vinegar or citrus fruits (acid-base adjustments). If it comes into contact with your eyes, wash it with running water. 

- Store in a cool dry place.

- Keep away from direct sunlight.

- Keep away from children.

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Citric Acid/CA

Chemicals

Specifications:
- USP Grade
- pH Down Solution
- Fine white powder, odorless.
- Available in 120 ml diluted pH adjuster and 60 g powder, good for 1 litre.
- Store in a cool dry place.
- Keep away from direct sunlight.
- Keep away from children.

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Copper Sulfate

Chemicals

Specifications: 
- Technical Grade
- For plant tissue culture use, it acts as a protectant for possible contaminants (e.g. bacteria, fungi, and dust mites) if concentrated. 
- Fine blue powder, odorless.
- Available in 120 ml concentrated solution and 250 g powder, good for 1 litre.
- Store in a cool dry place.
- Keep away from direct sunlight.
- Keep away from children.

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Modified Knudson C

Chemicals

Specifications:
- Complete fertilizer mix for plant tissue culture.
- Available in 50 g pack, good for 20 litres. 
- Instructions attached inside.
- Can also be used as a foliar fertilizer.

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Agar Bar

Specifications:
- Pure white agar bar
- Sold per piece (9-10 grams)
- Solidifying agent for plant tissue culture medium.

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Autoclavable Glass Bottle

Specifications:
- 8 oz. 220 ml wide-mouth bottle.
Comes with autoclavable plastic cap.
- Sold per box of 24 bottles.

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Murashige & Skoog's Medium (MS Medium)

Specifications:

- Origin: China

- Complete nutrient basal medium with vitamins and Myo-Inositol.

- Hassle-free preparation - just add water then autoclave/sterilize

- Fixed pH 5.8

- Fixed sucrose 30g/liter

- Good for 6 liters

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pH Paper Strips

Specifications:

- Alkaline-Acid indicator paper

- Long-range pH test (from 0 to 14)

- 100 test strips/set

- Packing size: 88 x 67 x 10mm

- Weight: 45g


Application (Plant Tissue Culture):

Media preparation pH check. Can also be used in water quality, soil, aquarium, urine, saliva, etc.

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Weighing Scale

Specifications:
- Capacity: 1 gram – 10,000 grams
- Accurate to 1 gram
- Power Supply: 2 AA batteries included
- Low power consumption
- Auto zero – Auto-off
- Unit display: g/oz
- Auto switch off in 120 seconds without operation
- Size: 237 x 165mm

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Aseptic Culture Starter Kit

Includes:
- 100 ml 95% ethyl alcohol
- 120 ml 1N KOH
- 120 ml Citric Acid
- 120 ml Copper Sulfate
- 10 ml 6-BAP
- 10 ml ANAA
- 1 pack Modified Knudson-C (good for 20 litres)
- 1 Petri Dish
- 1 Long Forceps
- 1 Short Forceps
- 1 Scalpel Holder #4
- 5 Scalpel Blades #20/#22
- 1 Alcohol Lamp
- 1 Syringe
- 5 pH Strips with Indicator 
- FREE Roots Rooting Powder and USB LED Light

Tissue Culture Laboratory Equipment

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Transfer Case

A Formica-coated, wooden cabinet designed for transferring, cultivating, propagating, and/or inoculating any biological specimens - from micro-organisms such as bacteria, fungi (mushrooms) to cells and tissues of animals and plants - to prevent contamination. 

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The Harbest transfer case has a unique frontal parallel glass facing the worker with a 37" x 6" wide opening. An ideal design for positive airflow that protects the user from the samples and equipment inside. 

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Our transfer case is cost-efficient and helps with overall lab productivity. Its quality can compare with expensive stainless steel-type laminar flow hoods. 

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Blower Box

A heavy-duty vertical wooden box that releases positive air pressure out to the transfer cases. The lower part of the box has a protective fiber foam and UV-C Germicidal Lamp to pre-sanitize all possible contaminants (dust, microscopic mites, microbial spores) before it passes through the middle part of the box with H.E.P.A. (High-Efficiency Particulate Adsorption) Filter to absorb 99.97% of the particulates with the filtration efficiency of 0.3 μm which then blows positive airflow through the transfer case using a 2" turbo-type blower.

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It is designed to accommodate not only the transfer case but also the whole transfer/inoculating room. A single blower box can connect up to four transfer cases inside your laboratory. 

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